Antioxidant activity of plant extracts thesis

All the tests were performed in triplicate and the graph was plotted with the mean values.

antioxidant activity of plant extracts by dpph method

The scavenging activity was estimated based on the percentage of DPPH radical scavenged as the following equation: Scavenging. The samples were mixed well and then allowed to stand for 5 min at room temperature in order to allow complete reaction with the Folin-Ciocalteu reagent.

Abstract To investigate antioxidant potential of methanol extract of Alpinia nigra leaves.

in vitro antioxidant activity of plant extracts pdf

Fresh ABTS solution was prepared for each assay. The ingestion of natural antioxidants has been inversely associated with morbidity and mortality from degenerative disorders [ 6 ].

Antioxidant activity of plant extracts thesis

TFC was calculated by extrapolating the absorbance of reaction mixture on calibration curve of quercetin. Phytochemical screening Preliminary phytochemical screening of the A. Natural antioxidants either in the form of raw extracts or their chemical constituents are very effective to prevent the destructive processes caused by oxidative stress [ 2 ]. Nevertheless, it is still unknown whether A. It is not known which constituents of plant are associated in reducing the risk of chronic diseases, but antioxidants appear to play a major role in the protective effect of plant medicine. Methods The study was done by using various in vitro methods such as 1,1-diphenylpicrylhydrazyl DPPH , 2,2-azino-bis 3-ethylbenzthiazolinesulphonic acid ABTS , nitric oxide and hydrogen peroxide radical scavenging assays. The search for novel natural antioxidants of plant origin has ever since increased. Different parts such as seeds, leaves and bark of stem and root known to contain substantial amounts of phytoconstituents such as phenolics, flavonoids, tannins having the ability to inhibit the free radicals that are excessively produced, hence can act as antioxidants [4]. Open Peer Review reports Background Since very old times, herbal medications have been used for relief of symptoms of disease [ 1 ]. All rights reserved. Keeping all these into account, the present study was undertaken to evaluate antioxidant activities as well as it deals with preliminary phytochemical screening, total phenolics and total flavonoid content TFC. The antioxidant contents of medicinal plants may contribute to the protection they offer from disease. The leaves were taken from these plants, chopped into pieces and dried under shade for 10—15 d. Hydrogen peroxide scavenging activity Scavenging activity of hydrogen peroxide by the plant extract was estimated using the method of Ruch et al.

Conclusion Data from present results revealed that Torilis leptophylla act as an antioxidant agent due to its free radical scavenging and cytoprotective activity. Much interest, in medicinal plants however, emanates from their long use in folk medicines as well as their prophylactic properties, especially in developing countries.

in vitro antioxidant activity of medicinal plants pdf

Natural antioxidants either in the form of raw extracts or their chemical constituents are very effective to prevent the destructive processes caused by oxidative stress [ 2 ].

The methanol extract was filtered and then concentrated by using rotary evaporator to yield a semisolid mass

In vitro antioxidant activity thesis

The working solution was obtained by diluting DPPH solution with methanol to attain an absorbance of about 0. Natural antioxidants either in the form of raw extracts or their chemical constituents are very effective to prevent the destructive processes caused by oxidative stress [ 2 ]. Alpinia nigra B. Despite the great advances observed in modern medicine in recent decades, plants still make an important contribution to health care. Preliminary phytochemical screening The leaf extract of A. Materials and methods 2. Extract preparation The fresh, whole plant 3 kg was collected and shade dried to obtain g dry sample which was later coarsely powdered in a Willy Mill to mesh size and used for solvent extraction. The absorbance of the solution was taken at nm against a blank solution containing the plant extract in PBS without H2O2. Ascorbic acid was used as positive control. Then 2. The resulting solution was then diluted by mixing 1 mL of freshly prepared ABTS solution to obtain an absorbance of 0.
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